Bufotenine (5-HO-DMT, or 5-hydroxy-N,N-dimethyltryptamine) and other related 5-hydroxy-indolethylamines are common constituents in the venom of several toad families, most notably Hyla, Leptodactylus, Rana and Bufo. The best known of these is probably Bufo Alvarius, the Colorado River Toad.

Bufotenine is not psychoactive in "reasonable doses", but with higher doses dangerous physiological side effects can manifest.

The safest way to consume it is to collect the venom from the toad in question, e.g. on a glass plate. The venom is viscous and milky-white in color when first squeezed from the glands. It begins to dry within minutes and acquires the color and texture of rubber cement. Scrape the venom from the glass plate, dry it thoroughly, and store it in an airtight container until you are ready to smoke it.

As with other tryptamines, the effects when smoked is almost instantaneous, and can be felt already after the first inhalation. All effects are usually completely gone within one hour of intake.