The longest scene in Shakespeare is Hamlet: Act I, Scene I.

In Real Time, it only goes for about fifteen minutes. In the play, it goes from midnight till daybreak.

Note that Bernardo says

'Tis now struck twelve,

that is, it is now midnight.

Later in the scene, the cock crows, which

Doth with his lofty and shrill-sounding throat / Awake the god of day.

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