The Ladies are famous for their live shows, in which they do a lot of improv and comedy. Songs are tweaked slightly, or greatly, lyrically to suit the news of the day or some random thought that crosses the mind of Ed Robertson or Steven Paige. The changes are often subtle (the joys of live taping, you can catch things that you couldn't even hear when it was happening), like in "These Apples" they'll sing "and mix up my grandma" instead of grammer. They also have a tendency to come up with new songs on the spot usually based on some slip of the tongue or odd phrase or news story.

The medley section is also popular, in which they do a medley of popular songs of the day, often to highly comedic effect. They really do put a lot of heart into the section, which is what makes it so great, but Steve trying to sing "And I will always love you" and hit (and sustain) those notes is hilarious.

On occasion they'll play an obscure unreleased track like "McDonald's Girl" or "Steven Page Is Having A Baby"