In the Vampire: The Masquerade role-playing game, Sanguinus is the unique Discipline of the Blood Brothers bloodline. The Blood Brothers, a vampire bloodline created as a result of experimentation by Tzimisce fleshcrafters and Koldunic sorcerors, with the assistance of the Tremere antitribu, are primarily intended for physical combat, much as Virstania's Gargoyles were intended during the early days of Clan Tremere. Blood Brothers work in collective packs, with a group mind directing their actions.

Blood Brother collectives use Sanguinus to coordinate their actions, share blood and thought, and at higher levels can use it to combine their very bodies to form massive, powerful war-forms. Like war ghouls, but much, much worse.