I have heard this word pronounced two different ways, and both equally common. In my experience, it seems that computer scientists like to pronounce it "TOO - pul", whereas mathematicians like to pronounce it "TUH - pul".

According to Webster, the former is correct.

TUPLE is also the name of a programming language/development environment, a parallel LISP based on KCL. It stands for "Toyohashi University Parallel Lisp Environment". (This last definition I discovered at Dictionary.com)