A form of
electrophoresis in which a gel or membrane is run in one direction, rotated 90
o, and run again under a different set of conditions (so as to gain a different separation of components). 2D gel electrophoresis is a powerful technique for protein separation, purification, and analysis.
first run
--------->
_________________ _________________
| * * * * | | * |
| | | * * | |
| | | * | |
| | | * | |second
| | | | | run
| | | * | |
| | | * | v
|_______________| |_______________|
Also written '2D electrophoresis'.