is a laboratory technique
used by researcher
s to figure out the order of the amino acids
in a polypeptide
(a chain of amino acid
It involves using the Edman reagent, phenyl isothiocyanate (PITC), to react one by one with each amino acid, in order. The technique is used in machines which automatically sequence (determine the order of subunits) polypeptides.
From the BioTech Dictionary at http://biotech.icmb.utexas.edu/. For further information see the BioTech homenode.