Heine Brothers Coffee is a franchised coffee shop centralised in Louisville, Kentucky, USA. It was started in 1992 in a grocery store, and the first location opened on Bardstown Road in 1994. The other branches opened in 1995, 1997, 1999, and 2000. The business is owned by Gary Heine and Mike Mays.

The Heine Brothers Coffee attracts locals of Louiseville, and the marketing is focused on familiarity and the magic of everyday living. Their signs are written in a Victorian font, yellow and white on a black field. The stores open at 7 o'clock in the morning. Every Thursday evening, at the original location, there is a bluegrass band which plays.

They sell Fair Trade, Organic, and Shade-Grown coffees, Blenheim's Ginger Ale, and Kizito cookies. One can buy coffee from them online, or order by telephone. Also, they are among the gay-friendly businesses in the Louisville area.