Maltose consists of two glucose molecules linked by 1,4 glycosidic bonds. This bonds forms by condensation reaction, during which an H and an OH group on two glucose molecules combine to form water, leaving the glucose molecules bonded together.

In the mouth glucose is produced by the action of salivary amylase (an enzyme) on starch in the food. Amylase is also produced by the pancreas and released into the ilium, part of the small intestine, to further break down starch into maltose. Maltose is then acted upon by maltase to produce glucose, which is small enough to be absorbed by the lining of the intestine.