How to do a Gram Stain (idea)
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Sat Nov 25 2000 at 15:51:34
Heat-fix a smear of the
on a slide by passing the slide briefly through a small flame.
Apply crystal violet dye to the stain and wash off after 30 seconds. This is the primary stain.
Cover the rinsed slide with
, which is a mordans and will help crystalize the violet in the cells., after 30 sec, rinse off the iodine.
Wash the slide in
to decolorize the slide.
Stain with a dark red solution of
which will serve as a counter stain.
Observe under a
, the bacteria which remained purple after the alcohol rinse is Gram-positive, the bacteria that is dark red (from the safarin) stain is Gram-negative.
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