Tito Puente is featured in the "Who shot Mr. Burns" two part saga in the Simpsons. He's part of Lisa Simpson's request for a jazz program when oil is discovered under Springfield Elementary. Because of Mr. Burns' slant drilling operation, the jazz program is cancelled resulting in his firing. This causes police chief Clancy Wiggum to declare him a suspect in the attempted murder of Mr. Burns.

Memorable quotes:
Tito Puente: "Why wound his body with bullets when I can set his soul afire with a slanderous mambo? Listen, if you will, to my revenge."
(song)
Clancy Wiggum: "Wow! Well, I believe you're not guilty. Gee, I hope all our suspects are this entertaining."