term that refers to a non-covalent
interaction involving a fluorophore
and another molecule creating a complex which can no longer fluoresce
If you are a quencher, there are two ways you can induce a fluorophore not to emit light. You can collide with it while its in the excited state, thereby taking away its energy. This is known as dynamic quenching. Dynamic quenching may occur through the formation of an exciplex. Otherwise, you may bind to the fluorophore in such a way that when it is hit by light, it can no longer fluoresce. This is known as static quenching. The complex of the fluorophore and quencher in this case is a dark complex. Dark complexes often have different ground state electronic properties which can be visualized by a change in the absorption spectrum.